Anti-Human RHEB Polyclonal Antibody (HS914014)

Name: Anti-Human RHEB Polyclonal Antibody
Brand: abinScience
SKU: HS914014
Price: 168 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HS914014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RHEB (Met1-Met184).

Target

RHEB2, Ras homolog enriched in brain, RHEB, GTP-binding protein Rheb

Purification

Purified by antigen affinity column.

Accession

Q15382

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

GTP-binding protein Rheb is a ~20 kDa protein. Small GTPase that acts as an allosteric activator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. In response to nutrients, growth factors or amino acids, specifically activates the protein kinase activity of MTOR, the catalytic component of the mTORC1 complex: acts by causing a conformational change that allows the alignment of residues in the active site of MTOR, thereby enhancing the phosphorylation of ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1). RHEB is also required for localization of the TSC-TBC complex to lysosomal membranes. In response to starvation, RHEB is inactivated by the TSC-TBC complex, preventing activation of mTORC1. Has low intrinsic GTPase activity.

1. Inoki, K. et al. (2002) Nature cell biology 4, 648-57. PMID: 12172553

2. Tee, AR. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 13571-6. PMID: 12271141

3. Castro, AF. et al. (2003) The Journal of biological chemistry 278, 32493-6. PMID: 12842888

4. Inoki, K. et al. (2003) Genes & development 17, 1829-34. PMID: 12869586

5. Tee, AR. et al. (2003) Current biology : CB 13, 1259-68. PMID: 12906785

6. Li, Y. et al. (2004) Molecular and cellular biology 24, 7965-75. PMID: 15340059

7. Long, X. et al. (2005) Current biology : CB 15, 702-13. PMID: 15854902

8. Tee, AR. et al. (2005) FEBS letters 579, 4763-8. PMID: 16098514

9. Sancak, Y. et al. (2010) Cell 141, 290-303. PMID: 20381137

10. Mazhab-Jafari, MT. et al. (2012) Structure (London, England : 1993) 20, 1528-39. PMID: 22819219

Note

For research use only.

Images

References

Datasheet

Anti-Human RHEB Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human RHEB Polyclonal Antibody (HS914014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us