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Anti-Human OS9 Polyclonal Antibody (HC357014)

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Overview
Catalog No.HC357014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human OS9 (Asn32-Gln251).
Target Protein OS-9, OS9, Amplified in osteosarcoma 9
Purification Purified by antigen affinity column.
Accession Q13438
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Protein OS-9 (OS9) is a ~75 kDa protein. Lectin component of the HRD1 complex, which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). Specifically recognizes and binds improperly folded glycoproteins as well as hyperglycosylated proteins, retain them in the ER, and transfers them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4 as well as hyperglycosylated HSP90B1.

1. Christianson, JC. et al. (2008) Nature cell biology 10, 272-82. PMID: 18264092
2. Bernasconi, R. et al. (2008) The Journal of biological chemistry 283, 16446-54. PMID: 18417469
3. Alcock, F. et al. (2009) Journal of molecular biology 385, 1032-42. PMID: 19084021
4. Hosokawa, N. et al. (2009) The Journal of biological chemistry 284, 17061-17068. PMID: 19346256
5. Satoh, T. et al. (2010) Molecular cell 40, 905-16. PMID: 21172656
6. Dersh, D. et al. (2014) Molecular biology of the cell 25, 2220-34. PMID: 24899641
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human OS9 Polyclonal Antibody

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