Anti-TOP2A Polyclonal Antibody (HY175024)

Name: Anti-TOP2A Polyclonal Antibody
Brand: abinScience
SKU: HY175024
Price: 168 USD
Availability: InStock

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Price(USD): $

Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

HY175024

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TOP2A (His54-Lys76).

Target

DNA topoisomerase II, alpha isozyme, TOP2A, DNA topoisomerase 2-alpha, TOP2

Purification

Purified by antigen affinity column.

Accession

P11388

RRID

Anti-TOP2A Polyclonal Antibody (abinScience Cat# HY175024, RRID:AB_3730954)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

DNA topoisomerase 2-alpha (TOP2A) is a ~174 kDa protein. Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand. May play a role in regulating the period length of BMAL1 transcriptional oscillation.

1. Wyles, JP. et al. (2007) Nucleic acids research 35, 4076-85. PMID: 17567603

2. Williamson, EA. et al. (2008) Nucleic acids research 36, 5822-31. PMID: 18790802

3. Ramamoorthy, M. et al. (2012) Nucleic acids research 40, 1621-35. PMID: 22013166

4. Lee, S. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 2925-30. PMID: 22323612

5. Kratz, AS. et al. (2016) Cell cycle (Georgetown, Tex.) 15, 3419-3431. PMID: 27754753

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-TOP2A polyclonal antibody at 1ug/mL on recombinant TOP2A (Catalog No. HY175022).

References

Datasheet

Anti-TOP2A Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-TOP2A Polyclonal Antibody (HY175024)

Overview

Images

References

Datasheet

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