Anti-Human ACAD9 Polyclonal Antibody (HP164014)

Name: Anti-Human ACAD9 Polyclonal Antibody
Brand: abinScience
SKU: HP164014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HP164014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ACAD9 (Thr11-Asn429).

Target

ACAD9, Acyl-CoA dehydrogenase family member 9, ACAD-9, Complex I assembly factor ACAD9, mitochondrial

Purification

Purified by antigen affinity column.

Accession

Q9H845

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Complex I assembly factor ACAD9, mitochondrial is a ~68 kDa protein. Together with NDUFAF1 and ECSIT, forms part of the mitochondrial complex I (MCIA),which is required for the biogenesis of respiratory Complex I (CI) and is therefore crucial for the activation of the oxidative phosphorylation system. ECSIT binding triggers a large conformational change, switching ACAD9 from a fatty acid oxidation (FAO) enzyme to a CI assembly factor. The function in CI assembly is independent of the FAO activity of the protein. As FAO enzyme, it catalyzes the first step in FAO, which consists in the proR-proR stereospecific alpha, beta-dehydrogenation of fatty acyl-CoA thioesters using the electron transfer flavoprotein (ETF) as their physiologic electron acceptor, resulting in the formation of trans-2-enoyl-CoA ((2E)-enoyl-CoA). Its preferred substrates are both saturated and unsaturated long-chain acyl-CoA substrates, with optimum activity toward the latter.

1. Nouws, J. et al. (2010) Cell metabolism 12, 283-94. PMID: 20816094

2. Nouws, J. et al. (2014) Human molecular genetics 23, 1311-9. PMID: 24158852

3. Formosa, LE. et al. (2020) Cell reports 31, 107541. PMID: 32320651

4. McGregor, L. et al. (2023) Nature communications 14, 8248. PMID: 38086790

5. Zhang, J. et al. (2002) Biochemical and biophysical research communications 297, 1033-42. PMID: 12359260

6. Ensenauer, R. et al. (2005) The Journal of biological chemistry 280, 32309-16. PMID: 16020546

7. He, M. et al. (2007) American journal of human genetics 81, 87-103. PMID: 17564966

8. He, M. et al. (2011) Molecular genetics and metabolism 102, 418-29. PMID: 21237683

Note

For research use only.

Images

References

Datasheet

Anti-Human ACAD9 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human ACAD9 Polyclonal Antibody (HP164014)

Overview

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Datasheet

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