Anti-Human BIRC7 Polyclonal Antibody (HP393014)

Name: Anti-Human BIRC7 Polyclonal Antibody
Brand: abinScience
SKU: HP393014
Price: 168 USD
Availability: InStock

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Price(USD): $

Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

HP393014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human BIRC7 (Ser86-Ser176).

Target

BIRC7, p30-Livin, Melanoma inhibitor of apoptosis protein, LIVIN, Baculoviral IAP repeat-containing protein 7, Kidney inhibitor of apoptosis protein, Truncated livin, RNF50, KIAP, Livin, RING-type E3 ubiquitin transferase BIRC7, RING finger protein 50, tLivin, MLIAP, ML-IAP

Purification

Purified by antigen affinity column.

Accession

Q96CA5

RRID

Anti-Human BIRC7 Polyclonal Antibody (abinScience Cat# HP393014, RRID:AB_3731138)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Baculoviral IAP repeat-containing protein 7 (BIRC7) is a ~32 kDa protein. Apoptotic regulator capable of exerting proapoptotic and anti-apoptotic activities and plays crucial roles in apoptosis, cell proliferation, and cell cycle control. Its anti-apoptotic activity is mediated through the inhibition of CASP3, CASP7 and CASP9, as well as by its E3 ubiquitin-protein ligase activity. As it is a weak caspase inhibitor, its anti-apoptotic activity is thought to be due to its ability to ubiquitinate DIABLO/SMAC targeting it for degradation thereby promoting cell survival. May contribute to caspase inhibition, by blocking the ability of DIABLO/SMAC to disrupt XIAP/BIRC4-caspase interactions. Protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine.

1. Kasof, GM. et al. (2001) The Journal of biological chemistry 276, 3238-46. PMID: 11024045

2. Vucic, D. et al. (2000) Current biology : CB 10, 1359-66. PMID: 11084335

3. Lin, JH. et al. (2000) Biochemical and biophysical research communications 279, 820-31. PMID: 11162435

4. Ma, L. et al. (2006) Cell death and differentiation 13, 2079-88. PMID: 16729033

5. Nachmias, B. et al. (2007) Apoptosis : an international journal on programmed cell death 12, 1129-42. PMID: 17294084

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human BIRC7 polyclonal antibody at 1ug/mL on recombinant Human BIRC7 (Catalog No. HP393012).

References

Datasheet

Anti-Human BIRC7 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human BIRC7 Polyclonal Antibody (HP393014)

Overview

Images

References

Datasheet

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