Anti-Human TDP2 Polyclonal Antibody (HF005014)

Name: Anti-Human TDP2 Polyclonal Antibody
Brand: abinScience
SKU: HF005014
Price: 168 USD
Availability: InStock

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Price(USD): $

Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

HF005014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TDP2 (Gly111-Leu362).

Target

Tyr-DNA phosphodiesterase 2, TDP2, hTDP2, 5'-tyrosyl-DNA phosphodiesterase, Tyrosyl-DNA phosphodiesterase 2, EAP2, Tyrosyl-RNA phosphodiesterase, EAPII, TTRAP, VPg unlinkase, 5'-Tyr-DNA phosphodiesterase, TRAF and TNF receptor-associated protein, ETS1-associated protein II, ETS1-associated protein 2

Purification

Purified by antigen affinity column.

Accession

O95551

RRID

Anti-Human TDP2 Polyclonal Antibody (abinScience Cat# HF005014, RRID:AB_3731217)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Tyrosyl-DNA phosphodiesterase 2 (TDP2) is a ~40 kDa protein. DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DNA double-strand breaks/DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Thereby, protects the transcription of many genes involved in neurological development and maintenance from the abortive activity of TOP2. Hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DSBs due to DNA damage by radiation and free radicals.

1. Schellenberg, MJ. et al. (2016) Nucleic acids research 44, 3829-44. PMID: 27060144

2. Hornyak, P. et al. (2016) The Biochemical journal 473, 1869-79. PMID: 27099339

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human TDP2 polyclonal antibody at 1ug/mL on recombinant Human TDP2 (Catalog No. HF005012).

References

Datasheet

Anti-Human TDP2 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human TDP2 Polyclonal Antibody (HF005014)

Overview

Images

References

Datasheet

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