Anti-Human RBPMS Polyclonal Antibody (HP322014)

Name: Anti-Human RBPMS Polyclonal Antibody
Brand: abinScience
SKU: HP322014
Price: 168 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

HP322014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RBPMS (Glu22-Glu97).

Target

Hermes, Heart and RRM expressed sequence, RNA-binding protein with multiple splicing, HERMES, RBP-MS, RBPMS

Purification

Purified by antigen affinity column.

Accession

Q93062

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

RNA-binding protein with multiple splicing (RBPMS) is a ~21 kDa protein. RNA binding protein that mediates the regulation of pre-mRNA alternative splicing (AS). Acts either as activator (FLNB, HSPG2, LIPA1, MYOCD, PTPRF and PPFIBP1) or repressor (TPM1, ACTN1, ITGA7, PIEZO1, LSM14B, MBNL1 and MBML2) of splicing events on specific pre-mRNA targets. Together with RNA binding proteins RBFOX2 and MBNL1/2, activates a splicing program associated with differentiated contractile vascular smooth muscle cells (SMC) by regulating AS of numerous pre-mRNA involved in actin cytoskeleton and focal adhesion machineries, suggesting a role in promoting a cell differentiated state. Binds to introns, exons and 3'-UTR associated with tandem CAC trinucleotide motifs separated by a variable spacer region, at a minimum as a dimer. The minimal length of RNA required for RBPMS-binding tandem CAC motifs is 15 nt, with spacing ranging from 1 to 9 nt.

1. Farazi, TA. et al. (2014) RNA (New York, N.Y.) 20, 1090-102. PMID: 24860013

2. Teplova, M. et al. (2016) Quarterly reviews of biophysics 49, e1. PMID: 26347403

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human RBPMS polyclonal antibody at 1ug/mL on recombinant Human RBPMS (Catalog No. HP322012).

References

Datasheet

Anti-Human RBPMS Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human RBPMS Polyclonal Antibody (HP322014)

Overview

Images

References

Datasheet

SDS

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