Anti-Human CD148/PTPRJ Polyclonal Antibody (HC363014)

Name: Anti-Human CD148/PTPRJ Polyclonal Antibody
Brand: abinScience
SKU: HC363014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HC363014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD148/PTPRJ (Arg997-Ala1337).

Target

HPTP eta, R-PTP-J, Protein-tyrosine phosphatase eta, Density-enhanced phosphatase 1, Receptor-type tyrosine-protein phosphatase eta, Protein-tyrosine phosphatase receptor type J, DEP-1, R-PTP-eta, CD148, PTPRJ, DEP1

Purification

Purified by antigen affinity column.

Accession

Q12913

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Receptor-type tyrosine-protein phosphatase eta (CD148/PTPRJ) is a ~145 kDa protein. Tyrosine phosphatase which dephosphorylates or contributes to the dephosphorylation of CTNND1, FLT3, PDGFRB, MET, KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2. Plays a role in cell adhesion, migration, proliferation and differentiation. Has a role in megakaryocytes and platelet formation. Involved in vascular development. Regulator of macrophage adhesion and spreading.

1. Kovalenko, M. et al. (2000) The Journal of biological chemistry 275, 16219-26. PMID: 10821867

2. Persson, C. et al. (2002) FEBS letters 517, 27-31. PMID: 12062403

3. Holsinger, LJ. et al. (2002) Oncogene 21, 7067-76. PMID: 12370829

4. Palka, HL. et al. (2003) The Journal of biological chemistry 278, 5728-35. PMID: 12475979

5. Tsuboi, N. et al. (2008) The Biochemical journal 413, 193-200. PMID: 18348712

6. Sacco, F. et al. (2009) The Journal of biological chemistry 284, 22048-22058. PMID: 19494114

7. Omerovic, J. et al. (2010) The Biochemical journal 426, 65-72. PMID: 19922411

8. Arora, D. et al. (2011) The Journal of biological chemistry 286, 10918-29. PMID: 21262971

9. Kellie, S. et al. (2004) Journal of cell science 117, 609-18. PMID: 14709717

Note

For research use only.

Images

References

Datasheet

Anti-Human CD148/PTPRJ Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human CD148/PTPRJ Polyclonal Antibody (HC363014)

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