Anti-CSPG4 Polyclonal Antibody (HV349014)

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Overview

Catalog No.	HV349014
Species reactivity	Human, Mouse, Rat
Applications	ELISA, IHC, WB
Host species	Rabbit
Isotype	IgG
Clonality	Polyclonal
Immunogen	E. coli - derived recombinant Human CSPG4 (Ser1705-Ile1942).
Target	Chondroitin sulfate proteoglycan 4, CSPG4, Chondroitin sulfate proteoglycan NG2, MCSP, Melanoma chondroitin sulfate proteoglycan, Melanoma-associated chondroitin sulfate proteoglycan
Purification	Purified by antigen affinity column.
Accession	Q6UVK1
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note	For research use only.

Images

Immunofluorescence

U2OS cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting CSPG4 (HV349014) at a concentration of 28 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of CSPG4 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

References

Datasheet