Anti-CHOP/GADD153/DDIT3 Polyclonal Antibody (HW574014)

Name: Anti-CHOP/GADD153/DDIT3 Polyclonal Antibody
Brand: abinScience
SKU: HW574014
Price: 168 USD
Availability: InStock

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100ug

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HW574014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CHOP/GADD153/DDIT3 (Arg105-Ala169).

Target

C/EBP-homologous protein, DDIT3, DDIT-3, CCAAT/enhancer-binding protein homologous protein, C/EBP zeta, CHOP-10, Growth arrest and DNA damage-inducible protein GADD153, DNA damage-inducible transcript 3 protein, GADD153, CHOP10, C/EBP-homologous protein 10, CHOP

Purification

Purified by antigen affinity column.

Accession

P35638

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

DNA damage-inducible transcript 3 protein (CHOP/GADD153) is a ~19 kDa protein. Multifunctional transcription factor in endoplasmic reticulum (ER) stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L.

1. Yamaguchi, H. et al. (2004) The Journal of biological chemistry 279, 45495-502. PMID: 15322075

2. Ohoka, N. et al. (2005) The EMBO journal 24, 1243-55. PMID: 15775988

3. Oliveira, SJ. et al. (2009) PloS one 4, e6618. PMID: 19672300

4. Vij, N. et al. (2008) American journal of respiratory cell and molecular biology 38, 176-84. PMID: 17709599

5. Goodall, JC. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 17698-703. PMID: 20876114

6. Ghosh, AP. et al. (2012) PloS one 7, e39586. PMID: 22761832

7. Su, N. et al. (2008) The Journal of biological chemistry 283, 35106-17. PMID: 18940792

8. Park, SH. et al. (2010) The Journal of biological chemistry 285, 35330-9. PMID: 20829347

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CHOP/GADD153/DDIT3 polyclonal antibody at 1ug/mL on recombinant CHOP/GADD153/DDIT3 (Catalog No. HW574022).

References

Datasheet