Anti-CD282/TLR2 Polyclonal Antibody (HT188014)

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50ug
100ug

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Overview
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Datasheet

Overview

Catalog No.

HT188014

Description

Anti-CD282/TLR2 Polyclonal Antibody (HT188014) is a rabbit polyclonal antibody detecting CD282 in ELISA, IHC, WB. Suitable for Human, Macaca fascicularis, Macaca mulatta, Pan paniscus, and Pan troglodytes.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Gorilla gorilla gorilla, Macaca fascicularis, Macaca mulatta, Pan paniscus, Pan troglodytes

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD282/TLR2 (Glu21-Leu590).

Target

TIL4, Toll-like receptor 2, TLR2, Toll/interleukin-1 receptor-like protein 4, CD282

Purification

Purified by antigen affinity column.

Accession

O60603

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Toll-like receptor 2 (CD282/TLR2) is a ~89 kDa protein. Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also activate immune cells and promote apoptosis in response to the lipid moiety of lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.

1. Jin, MS. et al. (2007) Cell 130, 1071-82. PMID: 17889651

2. Lancioni, CL. et al. (2011) Infection and immunity 79, 663-73. PMID: 21078852

3. Brightbill, HD. et al. (1999) Science (New York, N.Y.) 285, 732-6. PMID: 10426995

4. Aliprantis, AO. et al. (1999) Science (New York, N.Y.) 285, 736-9. PMID: 10426996

5. Bulut, Y. et al. (2001) Journal of immunology (Baltimore, Md. : 1950) 167, 987-94. PMID: 11441107

6. Jung, SB. et al. (2006) Infection and immunity 74, 2686-96. PMID: 16622205

7. Drage, MG. et al. (2009) Cellular immunology 258, 29-37. PMID: 19362712

8. Bulut, Y. et al. (2005) The Journal of biological chemistry 280, 20961-7. PMID: 15809303

9. Triantafilou, M. et al. (2006) The Journal of biological chemistry 281, 31002-11. PMID: 16880211

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CD282/TLR2 polyclonal antibody at 1ug/mL on recombinant CD282/TLR2 (Catalog No. HT188012).

References

Datasheet