Anti-Human Granzyme B Polyclonal Antibody (HY574024)

Name: Anti-Human Granzyme B Polyclonal Antibody
Brand: abinScience
SKU: HY574024
Price: 168 USD
Availability: InStock

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Overview
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SDS

Overview

Catalog No.

HY574024

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human GZMB (Ala18-Tyr247).

Target

Cathepsin G-like 1, Granzyme-2, GZMB, Fragmentin-2, Human lymphocyte protein, HLP, CTLA-1, CGL1, CTSGL1, CSPB, Lymphocyte protease, T-cell serine protease 1-3E, Granzyme B, CTLA1, GRB, C11, Cytotoxic T-lymphocyte proteinase 2, SECT

Purification

Purified by antigen affinity column.

Accession

P10144

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Granzyme B (GZMB) is a ~27 kDa protein. Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It cleaves after Asp. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -9 and -10 (CASP3, CASP9 and CASP10, respectively) to give rise to active enzymes mediating apoptosis.

1. Poe, M. et al. (1991) The Journal of biological chemistry 266, 98-103. PMID: 1985927

2. Hameed, A. et al. (1988) Journal of immunology (Baltimore, Md. : 1950) 141, 3142-7. PMID: 3262682

3. Krähenbühl, O. et al. (1988) Journal of immunology (Baltimore, Md. : 1950) 141, 3471-7. PMID: 3263427

4. Froelich, CJ. et al. (1993) Journal of immunology (Baltimore, Md. : 1950) 151, 7161-71. PMID: 8258716

5. Liu, Y. et al. (2020) Science immunology 5. PMID: 31953257

6. Zhang, Z. et al. (2020) Nature 579, 415-420. PMID: 32188940

7. Yang, X. et al. (1998) The Journal of biological chemistry 273, 34278-83. PMID: 9852092

Note

For research use only.

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References

Datasheet