Anti-Mouse CD244 Polyclonal Antibody (MV513014)

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Datasheet

Overview

Catalog No.

MV513014

Description

Anti-Mouse CD244 Polyclonal Antibody (MV513014) is a rabbit polyclonal antibody detecting CD244 in ELISA, IHC, WB. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Mouse CD244 (Asp24-Thr212).

Target

SLAM family member 4, SLAMF4, h2B4, Signaling lymphocytic activation molecule 4, Natural killer cell receptor 2B4, NKR2B4, NAIL, NK cell type I receptor protein 2B4, CD244, NK cell activation-inducing ligand, 2B4

Purification

Purified by antigen affinity column.

Accession

Q07763

RRID

Anti-Mouse CD244 Polyclonal Antibody (abinScience Cat# MV513014, RRID:AB_3723939)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Natural killer cell receptor 2B4 (CD244) is a ~44 kDa protein. Heterophilic receptor of the signaling lymphocytic activation molecule (SLAM) family; its ligand is CD48. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Acts as activating natural killer (NK) cell receptor. Activating function implicates association with SH2D1A and FYN.

1. Lee, KM. et al. (2003) Journal of immunology (Baltimore, Md. : 1950) 170, 4881-5. PMID: 12734329

2. Dong, Z. et al. (2009) Nature immunology 10, 973-80. PMID: 19648922

3. Wang, N. et al. (2010) Journal of immunology (Baltimore, Md. : 1950) 185, 5683-7. PMID: 20962259

4. Garni-Wagner, BA. et al. (1993) Journal of immunology (Baltimore, Md. : 1950) 151, 60-70. PMID: 8326140

5. Chen, R. et al. (2004) Molecular and cellular biology 24, 5144-56. PMID: 15169881

6. Bloch-Queyrat, C. et al. (2005) The Journal of experimental medicine 202, 181-92. PMID: 15998796

7. Dong, Z. et al. (2012) Immunity 36, 974-85. PMID: 22683124

8. Lee, KM. et al. (2006) Blood 107, 3181-8. PMID: 15905190

9. Roncagalli, R. et al. (2005) Nature immunology 6, 1002-10. PMID: 16127454

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CD244 polyclonal antibody at 1ug/mL on various samples.
Lane 1: MCF-7 cell lysate

References

Datasheet

Anti-Mouse CD244 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mouse CD244 Polyclonal Antibody (MV513014)

Overview

Images

References

Datasheet

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