Anti-SPARC/Osteonectin Polyclonal Antibody (HY111014)

Name: Anti-SPARC/Osteonectin Polyclonal Antibody
Brand: abinScience
SKU: HY111014
Price: 168 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HY111014

Description

Anti-SPARC/Osteonectin Polyclonal Antibody (HY111014) is a rabbit polyclonal antibody detecting Osteonectin in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SPARC/Osteonectin (Gln73-Lys279).

Target

Secreted protein acidic and rich in cysteine, Basement-membrane protein 40, BM-40, Osteonectin, ON, SPARC

Purification

Purified by antigen affinity column.

Accession

P09486

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

SPARC (Osteonectin) is a ~34 kDa protein. Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-SPARC/Osteonectin polyclonal antibody at 1ug/mL on recombinant SPARC/Osteonectin (Catalog No. HY111022).

References

Datasheet