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Anti-Corynephage omega DT/Diphtheria toxin Polyclonal Antibody (JN967014)

Anti-Corynephage omega DT/Diphtheria toxin Polyclonal Antibody
Anti-Corynephage omega DT/Diphtheria toxin Polyclonal Antibody
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Overview
Catalog No.JN967014
Description
Anti-Corynephage omega DT/Diphtheria toxin Polyclonal Antibody (JN967014) is a rabbit polyclonal antibody detecting Diphtheria toxin in ELISA, IHC, WB. Suitable for Corynephage omega.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityCorynephage omega
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Corynephage omega DT/Diphtheria toxin (Ala25-Ser560).
Target Diphtheria toxin, DT, NAD(+)--diphthamide ADP-ribosyltransferase, 2.4.2.36, Diphtheria toxin fragment A, Diphtheria toxin fragment B
Purification Purified by antigen affinity column.
Accession P00587
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Diphtheria toxin (DT) is a ~60 kDa protein. Diphtheria toxin, produced by a phage infecting Corynebacterium diphtheriae, is a proenzyme that, after activation, catalyzes the covalent attachment of the ADP ribose moiety of NAD to elongation factor 2. Fragment A is responsible for enzymatic ADP-ribosylation of elongation factor 2, while fragment B is responsible for binding of toxin to cell receptors and entry of fragment A.

Note For research use only.
Images
  • Anti-Corynephage omega DT/Diphtheria toxin Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-DT polyclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant Corynephage omega DT/Diphtheria toxin (Catalog No: JN967022)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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