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Anti-Human CD151 Polyclonal Antibody (HW567014)

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Overview
Catalog No.HW567014
Description
Anti-Human CD151 Polyclonal Antibody (HW567014) is a rabbit polyclonal antibody detecting CD151 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human CD151 (Ala113-Arg221).
Target CD151 antigen, GP27, Membrane glycoprotein SFA-1, Platelet-endothelial tetraspan antigen 3, PETA-3, Tetraspanin-24, Tspan-24, CD151, CD151, TSPAN24
Purification Purified by antigen affinity column.
Accession P48509
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

CD151 antigen is a ~28 kDa protein. Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Plays a role in various cellular and molecular mechanism through its association with both integrin and non-integrin proteins. These interactions facilitate critical cellular functions, including cell-to-cell communication, wound healing, platelet aggregation, trafficking, cell motility, and angiogenesis. Via interaction with JAM-A/F11R and integrin ITGA3:ITGB1, promotes the recruitment of signaling molecules such as RAC1, CDC42 and RhoGTPases to facilitate the polarization of epithelial cells and the reorganization of the actin cytoskeleton, which are critical steps in cell migration process. Regulates the glycosylation pattern of ITGA3:ITGB1 thereby modulating its activity.

1. Zheng, ZZ. et al. (2007) The international journal of biochemistry & cell biology 39, 340-8. PMID: 17045834
2. Rocha-Perugini, V. et al. (2014) European journal of immunology 44, 1967-75. PMID: 24723389
3. Te Molder, L. et al. (2019) Journal of cell science 132. PMID: 31488507
4. Hong, IK. et al. (2012) The Journal of biological chemistry 287, 32027-39. PMID: 22843693
5. Thölmann, S. et al. (2022) Cellular and molecular life sciences : CMLS 79, 88. PMID: 35067832
6. Baldwin, G. et al. (2008) The Journal of biological chemistry 283, 35445-54. PMID: 18852263
7. Karamatic Crew, V. et al. (2004) Blood 104, 2217-23. PMID: 15265795
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-Human CD151 Polyclonal Antibody

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