Anti-Human PEX12 Polyclonal Antibody (HT031014)

Name: Anti-Human PEX12 Polyclonal Antibody
Brand: abinScience
SKU: HT031014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HT031014

Description

Anti-Human PEX12 Polyclonal Antibody (HT031014) is a rabbit polyclonal antibody detecting PEX12 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PEX12 (Ala278-Asn359).

Target

Peroxisome assembly protein 12, Peroxin-12, Peroxisome assembly factor 3, PAF-3, PEX12, PAF3

Purification

Purified by antigen affinity column.

Accession

O00623

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Peroxisome assembly protein 12 (PEX12) is a ~40 kDa protein. Component of a retrotranslocation channel required for peroxisome organization by mediating export of the PEX5 receptor from peroxisomes to the cytosol, thereby promoting PEX5 recycling. The retrotranslocation channel is composed of PEX2, PEX10 and PEX12; each subunit contributing transmembrane segments that coassemble into an open channel that specifically allows the passage of PEX5 through the peroxisomal membrane. PEX12 also regulates PEX5 recycling by activating the E3 ubiquitin-protein ligase activity of PEX10. When PEX5 recycling is compromised, PEX12 stimulates PEX10-mediated polyubiquitination of PEX5, leading to its subsequent degradation.

1. Okumoto, K. et al. (2014) The Journal of biological chemistry 289, 14089-108. PMID: 24662292

2. Okumoto, K. et al. (1997) Nature genetics 17, 265-6. PMID: 9354782

3. Okumoto, K. et al. (1998) Molecular and cellular biology 18, 4324-36. PMID: 9632816

Note

For research use only.

Images

References

Datasheet

Anti-Human PEX12 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

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Anti-Human PEX12 Polyclonal Antibody (HT031014)

Overview

Images

References

Datasheet

SDS

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