Anti-Human USP37 Polyclonal Antibody (HA158014)

Name: Anti-Human USP37 Polyclonal Antibody
Brand: abinScience
SKU: HA158014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HA158014

Description

Anti-Human USP37 Polyclonal Antibody (HA158014) is a rabbit polyclonal antibody detecting USP37 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human USP37 (Met1-Arg106).

Target

Ubiquitin carboxyl-terminal hydrolase 37, 3.4.19.12, Deubiquitinating enzyme 37, Ubiquitin thioesterase 37, Ubiquitin-specific-processing protease 37, USP37, KIAA1594

Purification

Purified by antigen affinity column.

Accession

Q86T82

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Ubiquitin carboxyl-terminal hydrolase 37 (USP37) is a ~110 kDa protein. Deubiquitinase that plays a role in different processes including cell cycle regulation, DNA replication or DNA damage response. Antagonizes the anaphase-promoting complex (APC/C) during G1/S transition by mediating deubiquitination of cyclin-A (CCNA1 and CCNA2), thereby promoting S phase entry. Specifically mediates deubiquitination of 'Lys-11'-linked polyubiquitin chains, a specific ubiquitin-linkage type mediated by the APC/C complex. Phosphorylation at Ser-628 during G1/S phase maximizes the deubiquitinase activity, leading to prevent degradation of cyclin-A (CCNA1 and CCNA2). Plays an important role in the regulation of DNA replication by stabilizing the licensing factor CDT1.

1. Yeh, C. et al. (2015) Current biology : CB 25, 2290-9. PMID: 26299517

2. Hernández-Pérez, S. et al. (2016) Molecular oncology 10, 1196-206. PMID: 27296872

3. Xiao, Z. et al. (2019) American journal of cancer research 9, 2749-2759. PMID: 31911859

4. Stromberg, BR. et al. (2021) The Journal of biological chemistry 297, 101184. PMID: 34509474

5. Huang, X. et al. (2011) Molecular cell 42, 511-23. PMID: 21596315

6. Wu, C. et al. (2021) Nucleic acids research 49, 11224-11240. PMID: 34606619

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-USP37 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse spleen lysate

References

Datasheet

Anti-Human USP37 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human USP37 Polyclonal Antibody (HA158014)

Overview

Images

References

Datasheet

SDS

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