Anti-EBV/HHV-4 BZLF1 Polyclonal Antibody (VK536014)

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100ug

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Datasheet

Overview

Catalog No.

VK536014

Description

Anti-EBV/HHV-4 BZLF1 Polyclonal Antibody (VK536014) is a rabbit polyclonal antibody detecting BZLF1 in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant EBV/HHV-4 BZLF1 (Leu175-Phe245).

Target

Lytic switch protein BZLF1, EB1, Protein Z, Trans-activator protein BZLF1, Zta, bZIP transcription factor ZEBRA, BZLF1

Purification

Purified by antigen affinity column.

Accession

P03206

RRID

Anti-EBV/HHV-4 BZLF1 Polyclonal Antibody (abinScience Cat# VK536014, RRID:AB_3724919)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Lytic switch protein BZLF1 is a ~26 kDa protein. Transcription factor that acts as a molecular switch to induce the transition from the latent to the lytic or productive phase of the virus cycle (Probable). Mediates the switch from the latent to the lytic cycle of infection in cells containing a highly methylated viral genome. Probably binds to silenced chromatin and recruits host chromatin-remodeling enzymes. Regulates this switch by binding to 2 types of ZEBRA response elements (ZREs): the CpG-free AP-1 like elements (latency) and the methylated CpG-containing elements (lytic replication) (Probable). Activates preferentially the methylated forms of the viral lytic R (BRLF1) and Na (BRRF1) gene promoters, the latters being the first genes activated during Z-mediated reactivation in latently infected cells.

1. Schepers, A. et al. (1993) The EMBO journal 12, 3921-9. PMID: 8404860

2. Bhende, PM. et al. (2004) Nature genetics 36, 1099-104. PMID: 15361873

3. Bernaudat, F. et al. (2022) Nucleic acids research 50, 490-511. PMID: 34893887

4. Schaeffner, M. et al. (2019) Life science alliance 2. PMID: 30926617

5. Flower, K. et al. (2011) PloS one 6, e25922. PMID: 22022468

6. Dickerson, SJ. et al. (2009) PLoS pathogens 5, e1000356. PMID: 19325883

7. Feederle, R. et al. (2000) The EMBO journal 19, 3080-9. PMID: 10856251

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-EBV/HHV-4 BZLF1 polyclonal antibody at 1ug/mL on recombinant EBV/HHV-4 BZLF1 (Catalog No. VK536012).
Western blot

Western blot analysis was performed using anti-BLZF1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant EBV/HHV-4 BZLF1 (Catalog No: VK536012)

References

Datasheet

Anti-EBV/HHV-4 BZLF1 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

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Anti-EBV/HHV-4 BZLF1 Polyclonal Antibody (VK536014)

Overview

Images

References

Datasheet

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