Anti-dsDNA Recombinant Antibody (3E10#) (YP539203)

Name: Anti-dsDNA Recombinant Antibody (3E10#)
Brand: abinScience
SKU: YP539203
Price: 126 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

YP539203

Applications

DB, ELISA, IF

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

3E10#

Clonality

Monoclonal

Target

DNA, dsDNA

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

RRID

Anti-dsDNA Antibody (3E10#) (abinScience Cat# YP539203, RRID:AB_3725561)

Form

Liquid

Storage buffer

0.94 mM Citric acid monohydrate, 16.7mM Trisodium citrate dihydrate, 6.2% sucrose, pH 6.5, 0.01% Tween 80

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-dsDNA Antibody (3E10#).
Immunofluorescence

Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting dsDNA (YP539203) overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Mouse IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of dsDNA was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Immunofluorescence

MG63 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting dsDNA (YP539203) overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Mouse IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of dsDNA was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

References

Datasheet

Anti-dsDNA Recombinant Antibody (3E10#).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-dsDNA Recombinant Antibody (3E10#) (YP539203)

Overview

Images

References

Datasheet

SDS

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