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Anti-Phospho-CREB1 (pS119) Recombinant Antibody (P5.G10) (HB851023)

Anti-Phospho-CREB1 (pS119) Recombinant Antibody (P5.G10)
Anti-Phospho-CREB1 (pS119) Recombinant Antibody (P5.G10)
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Overview
Catalog No.HB851023
Species reactivityBovine
ApplicationsIHC, IP, WB
Host speciesMouse
IsotypeIgG2a, kappa
Clone IDP5.G10
Clonality Monoclonal
Target cAMP-responsive element-binding protein 1, CREB-1, CREB1, Cyclic AMP-responsive element-binding protein 1
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P16220
RRID Anti-Phospho-CREB1 (pS119) Antibody (P5.G10) (abinScience Cat# HB851023, RRID:AB_3725766)
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Cyclic AMP-responsive element-binding protein 1 (p-CREB1) is a ~35 kDa protein. Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-119 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells. Regulates the expression of apoptotic and inflammatory response factors in cardiomyocytes in response to ERFE-mediated activation of AKT signaling.

1. Conkright, MD. et al. (2003) Molecular cell 12, 413-23. PMID: 14536081
Note For research use only.
Images
  • Anti-Phospho-CREB1 (pS119) Recombinant Antibody (P5.G10)

    SDS-PAGE

    SDS-PAGE for Anti-Phospho-CREB1 (pS119) Antibody (P5.G10)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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