InVivoMAb Anti-Human CCL2/JE/MCP-1 Antibody (Iv0077) (HY280010)

Name: InVivoMAb Anti-Human CCL2/JE/MCP-1 Antibody (Iv0077)
Brand: abinScience
SKU: HY280010
Price: 370 USD
Availability: InStock

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Overview

Catalog No.	HY280010
Description	InVivoMAb Anti-Human CCL2/MCP-1 (Iv0077) [Iv0077] (HY280010) is a human monoclonal antibody detecting HC11 in ELISA, FCM, Neutralization. Suitable for Human. Highlights ●Multi-Application — Validated across multiple applications. ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	ELISA, WB, FCM, Neutralization
Host species	Human
Isotype	IgG1, kappa
Clone ID	Iv0077
Clonality	Monoclonal
Target	HC11, MCP-1, MCP1, Monocyte chemotactic protein 1, Monocyte chemotactic and activating factor, Small-inducible cytokine A2, SCYA2, MCAF, Monocyte secretory protein JE, C-C motif chemokine 2, Monocyte chemoattractant protein 1, CCL2
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P13500
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note	For research use only. Not suitable for clinical or therapeutic use.

Images

Bioactivity

SEC-HPLC detection for InVivoMAb Anti-Human CCL2/MCP-1 (ABV0269).
Flow Cytometry

Flow-cytometry using anti-human CCL2 antibody.A549 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CCL2 antibody monoclonal antibody (Catalog # HY280010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CCL2 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CCL2 antibody monoclonal antibody (Catalog # HY280010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for InVivoMAb Anti-Human CCL2/MCP-1 (Iv0077).
Western blot

Western blot analysis was performed using anti-CCL2/MCP-1 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human CCL2/MCP-1 (Catalog No: HY280012), 50ng
Lane 2: recombinant human CCL2/MCP-1 (Catalog No: HY280012), 30ng
Lane 3: recombinant human CCL2/MCP-1 (Catalog No: HY280012), 20ng
Lane 4: recombinant human CCL2/MCP-1 (Catalog No: HY280012), 10ng