InVivoMAb Anti-Human CCL2/MCP-1 Antibody (Iv0077) (HY280010)

Name: InVivoMAb Anti-Human CCL2/MCP-1 Antibody (Iv0077)
Brand: abinScience
SKU: HY280010
Price: 370 USD
Availability: InStock

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Overview

Catalog No.	HY280010
Description	InVivoMAb Anti-Human CCL2/MCP-1 (Iv0077) [Iv0077] (HY280010) is a human monoclonal antibody detecting HC11 in ELISA, FCM, Neutralization. Suitable for Human. Highlights ●Multi-Application — Validated across multiple applications. ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	ELISA, WB, FCM, Neutralization
Host species	Human
Isotype	IgG1, kappa
Clone ID	Iv0077
Clonality	Monoclonal
Target	HC11, MCP-1, MCP1, Monocyte chemotactic protein 1, Monocyte chemotactic and activating factor, Small-inducible cytokine A2, SCYA2, MCAF, Monocyte secretory protein JE, C-C motif chemokine 2, Monocyte chemoattractant protein 1, CCL2
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Associated products	pH 7.4 Dilution Buffer
Purification	Protein A/G purified from cell culture supernatant.
Accession	P13500
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

Images

Bioactivity

SEC-HPLC detection for InVivoMAb Anti-Human CCL2/MCP-1 (ABV0269).
Flow Cytometry

Flow-cytometry using anti-human CCL2 antibody.A549 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CCL2 antibody monoclonal antibody (Catalog # HY280010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CCL2 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CCL2 antibody monoclonal antibody (Catalog # HY280010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for InVivoMAb Anti-Human CCL2/MCP-1 (Iv0077).
Western blot

Western blot analysis was performed using anti-CCL2/MCP-1 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human CCL2/MCP-1 (Catalog No: HY280012), 50ng
Lane 2: recombinant human CCL2/MCP-1 (Catalog No: HY280012), 30ng
Lane 3: recombinant human CCL2/MCP-1 (Catalog No: HY280012), 20ng
Lane 4: recombinant human CCL2/MCP-1 (Catalog No: HY280012), 10ng