Anti-Human MC4R Recombinant Nanobody (SAA0796) (HB871013)

Name: Anti-Human MC4R Recombinant Nanobody (SAA0796)
Brand: abinScience
SKU: HB871013
Price: 126 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD): --

Spec:

20ug
50ug
100ug
1mg

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HB871013

Description

Anti-Human MC4R Nanobody (SAA0796) [SAA0796] (HB871013) is a alpaca monoclonal antibody detecting MC4R in Blocking, ELISA, FCM. Suitable for Human.

Highlights

●Multi-Application — Validated across multiple applications.
●Flow Cytometry — Validated for flow cytometry applications.

Species reactivity

Human

Applications

Blocking, ELISA, FCM

Host species

Alpaca

Clonality

Monoclonal

Isotype

VHH-8His-Cys-tag

Clone ID

SAA0796

Target

MC4R, MC4-R, Melanocortin receptor 4

Endotoxin level

< 10 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Accession

P32245

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Melanocortin receptor 4 (MC4R) is a ~36 kDa protein. G protein-coupled receptor that binds melanocyte-stimulating hormones (alpha- and beta-MSH) and corticotropin/ACTH, which are peptide products of the POMC precursor. Functions as a central component of the leptin-melanocortin pathway, which is essential for maintaining energy homeostasis. Upon activation, couples to G(s) protein, stimulating adenylate cyclase and the cAMP-dependent signaling pathway, which promotes anorexogenic signaling in the hypothalamus and contributes to a negative energy balance. Regulates food intake: activation by agonists suppresses appetite, whereas the antagonist Agouti-related protein/AGRP precludes agonist-induced signaling, thereby stimulating appetite. Modulates the firing activity of neurons in paraventricular nucleus (PVN) of the hypothalamus via alpha-MSH and AGRP regulation of inwardly rectifying potassium channel KCNJ13 closure, independently of G(s) signaling.

1. Farooqi, IS. et al. (2003) The New England journal of medicine 348, 1085-95. PMID: 12646665

2. Valli-Jaakola, K. et al. (2004) The Journal of clinical endocrinology and metabolism 89, 940-5. PMID: 14764818

3. Delhanty, PJ. et al. (2014) Molecular biology reports 41, 7967-72. PMID: 25163632

4. Yu, J. et al. (2020) Science (New York, N.Y.) 368, 428-433. PMID: 32327598

5. Israeli, H. et al. (2021) Science (New York, N.Y.) 372, 808-814. PMID: 33858992

6. Gantz, I. et al. (1993) The Journal of biological chemistry 268, 15174-9. PMID: 8392067

7. Yeo, GS. et al. (2003) Human molecular genetics 12, 561-74. PMID: 12588803

8. Ollmann, MM. et al. (1997) Science (New York, N.Y.) 278, 135-8. PMID: 9311920

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human MC4R Nanobody (SAA0796)

References

Datasheet

Anti-Human MC4R Recombinant Nanobody (SAA0796).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human MC4R Recombinant Nanobody (SAA0796) (HB871013)

Overview

Images

References

Datasheet

SDS