Anti-Human PTH1R Recombinant Nanobody (SAA0797) (HX947013)

Name: Anti-Human PTH1R Recombinant Nanobody (SAA0797)
Brand: abinScience
SKU: HX947013
Price: 126 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HX947013

Description

Anti-Human PTH1R Nanobody (SAA0797) [SAA0797] (HX947013) is a alpaca monoclonal antibody detecting PTH1R in ELISA, RIA. Suitable for Human.

Species reactivity

Human

Applications

ELISA, RIA, WB

Host species

Alpaca

Clonality

Monoclonal

Isotype

VHH-8His-Cys-tag

Clone ID

SAA0797

Tested applications

WB, IF

Target

PTH/PTHr receptor, Parathyroid hormone 1 receptor, PTHR1, PTHR, PTH1 receptor, PTH/PTHrP type I receptor, PTH1R, Parathyroid hormone/parathyroid hormone-related peptide receptor

Endotoxin level

< 10 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Accession

Q03431

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Parathyroid hormone/parathyroid hormone-related peptide receptor (PTH1R) is a ~66 kDa protein. G-protein-coupled receptor for parathyroid hormone (PTH) and for parathyroid hormone-related peptide (PTHLH). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (cAMP). PTH1R is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity. PTHLH dissociates from PTH1R more rapidly than PTH; as consequence, the cAMP response induced by PTHLH decays faster than the response induced by PTH.

1. Grauschopf, U. et al. (2000) Biochemistry 39, 8878-87. PMID: 10913300

2. Pioszak, AA. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 5034-9. PMID: 18375760

3. Pioszak, AA. et al. (2009) The Journal of biological chemistry 284, 28382-28391. PMID: 19674967

4. Shimomura-Kuroki, J. et al. (2017) Odontology 105, 150-154. PMID: 27160269

5. Zhao, LH. et al. (2019) Science (New York, N.Y.) 364, 148-153. PMID: 30975883

6. Kobayashi, K. et al. (2022) Molecular cell 82, 3468-3483.e5. PMID: 35932760

7. Schneider, H. et al. (1993) European journal of pharmacology 246, 149-55. PMID: 8397094

8. Pioszak, AA. et al. (2010) The Journal of biological chemistry 285, 12435-44. PMID: 20172855

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human PTH1R Nanobody (SAA0797)
Bioactivity

Detects recombinant PTH1R (Catalog No: HX947012) in indirect ELISA.
Western blot

Western blot analysis was performed using anti-PTH1R monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human PTH1R (Catalog No: HX947012)
Immunofluorescence

Immunofluorescence staining of MCF7 with Alpaca monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the Cell membrane .

References

Datasheet

Anti-Human PTH1R Recombinant Nanobody (SAA0797).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human PTH1R Recombinant Nanobody (SAA0797) (HX947013)

Overview

Images

References

Datasheet

SDS