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Anti-Human TG/Thyroglobulin Recombinant Nanobody (SAA1339) (HF743033)

Anti-Human TG/Thyroglobulin Recombinant Nanobody (SAA1339)
Anti-Human TG/Thyroglobulin Recombinant Nanobody (SAA1339)
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Overview
Catalog No.HF743033
Description
Anti-Human TG/Thyroglobulin Nanobody (SAA1339) [SAA1339] (HF743033) is a alpaca monoclonal antibody detecting Thyroglobulin in ELISA, IF, IHC, RIA, SPR. Suitable for Human.
Highlights
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IF, IHC, RIA, SPR
Host speciesAlpaca
ClonalityMonoclonal
IsotypeVHH-8His-Cys-tag
Clone IDSAA1339
Target Thyroglobulin, Tg, TG
Endotoxin level < 10 EU/mg as determined by LAL method.
Purity >95% as determined by SDS-PAGE.
Purification Purified by Nickel column.
Accession P01266
Form Liquid
Storage buffer 0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Thyroglobulin (TG) is a ~304 kDa protein. Acts as a substrate for the production of iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). The synthesis of T3 and T4 involves iodination of selected tyrosine residues of TG/thyroglobulin followed by their oxidative coupling in the thyroid follicle lumen. Following TG re-internalization and lysosomal-mediated proteolysis, T3 and T4 are released from the polypeptide backbone leading to their secretion into the bloodstream. One dimer produces 7 thyroid hormone molecules.

1. Caputo, M. et al. (2007) Clinical endocrinology 67, 351-7. PMID: 17532758
2. Coscia, F. et al. (2020) Nature 578, 627-630. PMID: 32025030
Note For research use only.
Images
  • Anti-Human TG/Thyroglobulin Recombinant Nanobody (SAA1339)

    SDS-PAGE

    SDS-PAGE for Anti-Human TG/Thyroglobulin Nanobody (SAA1339)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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