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Anti-Human Vimentin Recombinant Nanobody (SAA1226) (HY550053)

Anti-Human Vimentin Recombinant Nanobody (SAA1226)
Anti-Human Vimentin Recombinant Nanobody (SAA1226)
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Overview
Catalog No.HY550053
Description
Anti-Human VIM/Vimentin Nanobody (SAA1226) [SAA1226] (HY550053) is a alpaca monoclonal antibody detecting Vimentin in ELISA, IF, SPR, WB. Suitable for Human.
Highlights
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IF, SPR, WB
Host speciesAlpaca
ClonalityMonoclonal
IsotypeVHH-8His-Cys-tag
Clone IDSAA1226
Target Vimentin, VIM
Endotoxin level < 10 EU/mg as determined by LAL method.
Purity >95% as determined by SDS-PAGE.
Purification Purified by Nickel column.
Accession P08670
Form Liquid
Storage buffer 0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Vimentin is a ~53 kDa protein. Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front. Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner. May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips.

Note For research use only.
Images
  • Anti-Human Vimentin Recombinant Nanobody (SAA1226)

    SDS-PAGE

    SDS-PAGE for Anti-Human VIM/Vimentin Nanobody (SAA1226)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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