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Anti-Human ASIC1 Recombinant Nanobody (SAA1106) (HX873013)

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Anti-Human ASIC1 Recombinant Nanobody (SAA1106)
Anti-Human ASIC1 Recombinant Nanobody (SAA1106)
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Overview
Catalog No.HX873013
Description
Anti-Human ASIC1 Nanobody (SAA1106) [SAA1106] (HX873013) is a alpaca monoclonal antibody detecting ASIC1 in ELISA. Suitable for Human.
Species reactivityHuman
ApplicationsELISA
Host speciesAlpaca
ClonalityMonoclonal
IsotypeVHH-8His-Cys-tag
Clone IDSAA1106
Target ASIC1, Amiloride-sensitive cation channel 2, neuronal, BNaC2, Brain sodium channel 2, ACCN2, Acid-sensing ion channel 1, BNAC2
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Purified by Nickel column.
Accession P78348
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Acid-sensing ion channel 1 (ASIC1) is a ~59 kDa protein. Forms voltage-independent, pH-gated trimeric sodium channels that act as postsynaptic excitatory receptors in the nervous system, playing a crucial role in regulating synaptic plasticity, learning, and memory. Upon extracellular pH drop this channel elicits transient, fast activating, and completely desensitizing inward currents. Displays high selectivity for sodium ions but can also permit the permeation of other cations. Regulates more or less directly intracellular calcium concentration and CaMKII phosphorylation, and thereby the density of dendritic spines. Modulates neuronal activity in the circuits underlying innate fear.

1. Hoagland, EN. et al. (2010) The Journal of biological chemistry 285, 41852-62. PMID: 21036899
2. Sun, D. et al. (2020) eLife 9. PMID: 32915133
3. Wu, Y. et al. (2021) eLife 10. PMID: 34319232
Note For research use only.
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  • Anti-Human ASIC1 Recombinant Nanobody (SAA1106)

    SDS-PAGE

    SDS-PAGE for Anti-Human ASIC1 Nanobody (SAA1106)

References
Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human ASIC1 Recombinant Nanobody (SAA1106)

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