Anti-Human ATR Antibody (SAA1672) (HC586013)

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50ug
100ug
1mg

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Datasheet

Overview

Catalog No.

HC586013

Description

Anti-Human ATR Antibody (SAA1672) [SAA1672] (HC586013) is a mouse monoclonal antibody detecting ATR in ELISA. Suitable for Human.

Species reactivity

Human

Applications

ELISA

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

SAA1672

Clonality

Monoclonal

Target

FRP1, Serine/threonine-protein kinase ATR, FRAP-related protein 1, ATR, Ataxia telangiectasia and Rad3-related protein

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q13535

RRID

Anti-Human ATR Antibody (SAA1672) (abinScience Cat# HC586013, RRID:AB_3727433)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Serine/threonine-protein kinase ATR is a ~301 kDa protein. Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RBBP8, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination.

1. Hall-Jackson, CA. et al. (1999) Oncogene 18, 6707-13. PMID: 10597277

2. Kim, ST. et al. (1999) The Journal of biological chemistry 274, 37538-43. PMID: 10608806

3. Liu, Q. et al. (2000) Genes & development 14, 1448-59. PMID: 10859164

4. Cortez, D. et al. (2001) Science (New York, N.Y.) 294, 1713-6. PMID: 11721054

5. Zou, L. et al. (2003) Science (New York, N.Y.) 300, 1542-8. PMID: 12791985

6. Barr, SM. et al. (2003) Current biology : CB 13, 1047-51. PMID: 12814551

7. Wang, Y. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 15387-92. PMID: 14657349

8. Unsal-Kaçmaz, K. et al. (2004) Molecular and cellular biology 24, 1292-300. PMID: 14729973

9. Ward, IM. et al. (2004) The Journal of biological chemistry 279, 9677-80. PMID: 14742437

10. Cortez, D. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 10078-83. PMID: 15210935

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human ATR Antibody (SAA1672)

References

Datasheet

Anti-Human ATR Antibody (SAA1672).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human ATR Antibody (SAA1672) (HC586013)

Overview

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References

Datasheet

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