Anti-Human DDX4/Vasa Recombinant Antibody (SAA1970) (HV699013)

Name: Anti-Human DDX4/Vasa Recombinant Antibody (SAA1970)
Brand: abinScience
SKU: HV699013
Price: 196 USD
Availability: InStock

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100ug
1mg

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HV699013

Species reactivity

Human

Applications

ELISA

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

SAA1970

Clonality

Monoclonal

Target

DDX4, VASA, Vasa homolog, DEAD box protein 4, Probable ATP-dependent RNA helicase DDX4

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q9NQI0

RRID

Anti-Human DDX4/Vasa Antibody (SAA1970) (abinScience Cat# HV699013, RRID:AB_3727734)
_x005F_x000D_

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Probable ATP-dependent RNA helicase DDX4 (Vasa) is a ~79 kDa protein. ATP-dependent RNA helicase required during spermatogenesis. Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle. Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs.

1. Castrillon, DH. et al. (2000) Proceedings of the National Academy of Sciences of the United States of America 97, 9585-90. PMID: 10920202

2. Li, HJ. et al. (2010) Chinese medical journal 123, 2879-82. PMID: 21034600

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human DDX4/Vasa Antibody (SAA1970)

References

Datasheet

Anti-Human DDX4/Vasa Recombinant Antibody (SAA1970).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human DDX4/Vasa Recombinant Antibody (SAA1970) (HV699013)

Overview

Images

References

Datasheet

SDS

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