



| Catalog No. | HF879053 | ||||||
|---|---|---|---|---|---|---|---|
| Species reactivity | Human | ||||||
| Applications | ELISA, FCM, WB | ||||||
| Host species | Human | ||||||
| Isotype | IgG1, kappa | ||||||
| Clone ID | SAA2005 | ||||||
| Clonality | Monoclonal | ||||||
| Target | TNF, Tumor necrosis factor ligand superfamily member 2, N-terminal fragment, ICD2, NTF, TNF-a, TNF-alpha, Tumor necrosis factor, TNFSF2, TNFA, Cachectin, ICD1 | ||||||
| Endotoxin level | Please contact with the lab for this information. | ||||||
| Purity | >95% as determined by SDS-PAGE. | ||||||
| Purification | Protein A/G purified from cell culture supernatant. | ||||||
| Accession | P01375 | ||||||
| Form | Liquid | ||||||
| Storage buffer | 0.01M PBS, pH 7.4.                                                 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.  | 
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| Product Usage Information | 
                                            
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| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||||
| Note | For research use only. | 

Flow-cytometry using anti-human TNFa antibody.TNFa Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TNFa antibody monoclonal antibody (Catalog # HF879053 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for Anti-Human TNFa/TNF-alpha Antibody (SAA2005).
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