Anti-LRRK2/Dardarin Recombinant Antibody (1D8) (HV789013)

Name: Anti-LRRK2/Dardarin Recombinant Antibody (1D8)
Brand: abinScience
SKU: HV789013
Price: 196 USD
Availability: InStock

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50ug
100ug
1mg

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HV789013

Description

Anti-LRRK2/Dardarin Recombinant Antibody (1D8) (HV789013) is a mouse monoclonal antibody detecting LRRK2/Dardarin in IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Applications

IHC, WB

Host species

Mouse

Clonality

Monoclonal

Isotype

IgG2a, kappa

Clone ID

1D8

Target

Dardarin, Leucine-rich repeat serine/threonine-protein kinase 2, PARK8, LRRK2

Endotoxin level

< 10 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

RRID

Anti-LRRK2/Dardarin Antibody (1D8) (abinScience Cat# HV789013, RRID:AB_3728472)
_x005F_x000D_

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Leucine-rich repeat serine/threonine-protein kinase 2 (LRRK2/Dardarin) is a ~286 kDa protein. Serine/threonine-protein kinase which phosphorylates a broad range of proteins involved in multiple processes such as neuronal plasticity, innate immunity, autophagy, and vesicle trafficking. Is a key regulator of RAB GTPases by regulating the GTP/GDP exchange and interaction partners of RABs through phosphorylation. Phosphorylates RAB3A, RAB3B, RAB3C, RAB3D, RAB5A, RAB5B, RAB5C, RAB8A, RAB8B, RAB10, RAB12, RAB29, RAB35, and RAB43. Regulates the RAB3IP-catalyzed GDP/GTP exchange for RAB8A through the phosphorylation of 'Thr-72' on RAB8A. Inhibits the interaction between RAB8A and GDI1 and/or GDI2 by phosphorylating 'Thr-72' on RAB8A.

1. MacLeod, D. et al. (2006) Neuron 52, 587-93. PMID: 17114044

2. Zach, S. et al. (2010) PloS one 5, e13191. PMID: 20949042

3. Angeles, DC. et al. (2011) Human mutation 32, 1390-7. PMID: 21850687

4. Gómez-Suaga, P. et al. (2012) Human molecular genetics 21, 511-25. PMID: 22012985

5. MacLeod, DA. et al. (2013) Neuron 77, 425-39. PMID: 23395371

6. Piccoli, G. et al. (2014) Molecular and cellular biology 34, 2147-61. PMID: 24687852

7. Cho, HJ. et al. (2014) The EMBO journal 33, 2314-31. PMID: 25201882

8. Guerreiro, PS. et al. (2016) Molecular neurobiology 53, 3124-3135. PMID: 26014385

9. Steger, M. et al. (2016) eLife 5. PMID: 26824392

10. Yan, R. et al. (2017) Protein & cell 8, 55-66. PMID: 27830463

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-LRRK2/Dardarin Antibody (1D8)

References

Datasheet

Anti-LRRK2/Dardarin Recombinant Antibody (1D8).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-LRRK2/Dardarin Recombinant Antibody (1D8) (HV789013)

Overview

Images

References

Datasheet

SDS