Rat Anti-Human GPC3 Recombinant Antibody (YP7) (HW310053)

Name: Rat Anti-Human GPC3 Recombinant Antibody (YP7)
Brand: abinScience
SKU: HW310053
Price: 126 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HW310053

Species reactivity

Human

Applications

ELISA, FCM, WB

Host species

Rat

Isotype

IgG1, kappa

Clone ID

YP7

Clonality

Monoclonal

Target

GPC3, MXR7, GTR2-2, Glypican-3, Intestinal protein OCI-5, OCI5

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P51654

RRID

Rat Anti-Human GPC3 Antibody (YP7) (abinScience Cat# HW310053, RRID:AB_3729294)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Glypican-3 (GPC3/Glypican 3) is a ~65 kDa protein. Cell surface proteoglycan. Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins. Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signaling pathway.

1. De Cat, B. et al. (2003) The Journal of cell biology 163, 625-35. PMID: 14610063

2. Capurro, MI. et al. (2005) The Journal of biological chemistry 280, 41201-6. PMID: 16227623

3. Capurro, M. et al. (2014) Journal of cell science 127, 1565-75. PMID: 24496449

4. Davoodi, J. et al. (2007) Proteomics 7, 2300-10. PMID: 17549790

Note

For research use only.

Images

Bioactivity

Detects recombinant human Glypican-3/GPC3 (Cat No.: HW310011) in indirect ELISA.
Bioactivity

Detects recombinant mouse Glypican-3/GPC3 (Cat No.: MW310011) in indirect ELISA.
SDS-PAGE

SDS-PAGE for Rat Anti-Human GPC3 Antibody (YP7).
Flow CytoMetry

Flow-cytometry using anti-human GPC3 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human GPC3 monoclonal antibody (Catalog: HW310053) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Rat IgG H&L Polyclonal Antibody, FITC (abinScience: ？) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-GPC3 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human GPC3 (Catalog No: HW310011)
Lane 2: negative control