









| Catalog No. | HW310053 | ||||||
|---|---|---|---|---|---|---|---|
| Species reactivity | Human | ||||||
| Applications | ELISA, FCM, WB | ||||||
| Host species | Rat | ||||||
| Isotype | IgG1, kappa | ||||||
| Clone ID | YP7 | ||||||
| Clonality | Monoclonal | ||||||
| Target | GPC3, MXR7, GTR2-2, Glypican-3, Intestinal protein OCI-5, OCI5 | ||||||
| Endotoxin level | Please contact with the lab for this information. | ||||||
| Purity | >95% as determined by SDS-PAGE. | ||||||
| Purification | Protein A/G purified from cell culture supernatant. | ||||||
| Accession | P51654 | ||||||
| Form | Liquid | ||||||
| Storage buffer | 0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
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| Product Usage Information |
| ||||||
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||||
| Note | For research use only. |

Detects recombinant human Glypican-3/GPC3 (Cat No.: HW310011) in indirect ELISA.

Detects recombinant mouse Glypican-3/GPC3 (Cat No.: MW310011) in indirect ELISA.

SDS-PAGE for Rat Anti-Human GPC3 Antibody (YP7).

Flow-cytometry using anti-human GPC3 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human GPC3 monoclonal antibody (Catalog: HW310053) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Rat IgG H&L Polyclonal Antibody, FITC (abinScience: ?) and cells analysed on a NovoCyte Flow Cytometer.

Western blot analysis was performed using anti-GPC3 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human GPC3 (Catalog No: HW310011)
Lane 2: negative control




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