Anti-Human NDC80/HEC1 Antibody (9G3) (HT177013)

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50ug
100ug
1mg

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Datasheet

Overview

Catalog No.

HT177013

Description

Anti-Human NDC80/HEC1 Antibody (9G3) [9G3] (HT177013) is a mouse monoclonal antibody detecting NDC80 in FCM, ICC, IF, IP, WB. Suitable for Human.

Highlights

●Multi-Application — Validated across multiple applications.
●Flow Cytometry — Validated for flow cytometry applications.

Species reactivity

Human

Applications

FCM, ICC, IF, IP, WB

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

9G3

Clonality

Monoclonal

Target

Kinetochore protein NDC80 homolog, Highly expressed in cancer protein, Kinetochore protein Hec1, HsHec1, Kinetochore-associated protein 2, Retinoblastoma-associated protein HEC, NDC80, HEC, HEC1, KNTC2

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

O14777

RRID

Anti-Human NDC80/HEC1 Antibody (9G3) (abinScience Cat# HT177013, RRID:AB_3729529)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Kinetochore protein NDC80 homolog (HEC1) is a ~73 kDa protein. Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules.

1. Martin-Lluesma, S. et al. (2002) Science (New York, N.Y.) 297, 2267-70. PMID: 12351790

2. DeLuca, JG. et al. (2003) Current biology : CB 13, 2103-9. PMID: 14654001

3. Bharadwaj, R. et al. (2004) The Journal of biological chemistry 279, 13076-85. PMID: 14699129

4. Joseph, J. et al. (2004) Current biology : CB 14, 611-7. PMID: 15062103

5. Stucke, VM. et al. (2004) Chromosoma 113, 1-15. PMID: 15235793

6. Meraldi, P. et al. (2004) Developmental cell 7, 45-60. PMID: 15239953

7. DeLuca, JG. et al. (2005) Molecular biology of the cell 16, 519-31. PMID: 15548592

8. Lin, YT. et al. (2006) Oncogene 25, 6901-14. PMID: 16732327

9. Zhao, G. et al. (2019) The Journal of biological chemistry 294, 576-592. PMID: 30409912

10. Chen, Y. et al. (1997) Molecular and cellular biology 17, 6049-56. PMID: 9315664

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human NDC80/HEC1 Antibody (9G3)

References

Datasheet

Anti-Human NDC80/HEC1 Antibody (9G3).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human NDC80/HEC1 Antibody (9G3) (HT177013)

Overview

Images

References

Datasheet

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