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Anti-Human CD81 Antibody (005-C01) (HX855013)

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  • Datasheet

Overview
Catalog No.HX855013
Species reactivityHuman
ApplicationsELISA, FCM, IHC, WB
Host speciesHuman
IsotypeIgG1
Clone ID005-C01
Clonality Monoclonal
Target CD81 antigen, TSPAN28, Target of the antiproliferative antibody 1, Tspan-28, Tetraspanin-28, CD81, TAPA1, 26 kDa cell surface protein TAPA-1
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P60033
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note For research use only.
Images
  • SEC-HPLC

    SEC-HPLC detection for Anti-Human CD81 Antibody (005-C01).

  • Bioactivity

    Detects CD81 in indirect ELISAs.

  • Flow Cytometry

    Flow-cytometry using anti-human CD81 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD81 antibody monoclonal antibody (Catalog # HX855013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • SDS-PAGE

    SDS-PAGE for Anti-Human CD81 Antibody (005-C01).

  • Western blot

    Western blot analysis was performed using anti-CD81 monoclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant human CD81 (Catalog No: HX855011)
    Lane 2: negative control

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