Anti-Human WDR5 IP Recombinant Antibody (SAA0239) (HX013013)

Name: Anti-Human WDR5 IP Recombinant Antibody (SAA0239)
Brand: abinScience
SKU: HX013013
Price: 126 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

20ug
50ug
100ug
1mg

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HX013013

Species reactivity

Human

Applications

ELISA, FCM, IF, IP

Host species

Mouse

Isotype

IgG1

Clone ID

SAA0239

Clonality

Monoclonal

Target

WDR5, BMP2-induced 3-kb gene protein, WD repeat-containing protein 5, BIG3

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P61964

RRID

Anti-Human WDR5 IP Antibody (SAA0239) (abinScience Cat# HX013013, RRID:AB_3729773)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

WD repeat-containing protein 5 (WDR5) is a ~36 kDa protein. Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues.

1. Han, Z. et al. (2006) Molecular cell 22, 137-44. PMID: 16600877

2. Couture, JF. et al. (2006) Nature structural & molecular biology 13, 698-703. PMID: 16829960

3. Guelman, S. et al. (2009) Molecular and cellular biology 29, 1176-88. PMID: 19103755

4. Garapaty, S. et al. (2009) The Journal of biological chemistry 284, 7542-52. PMID: 19131338

5. Patel, A. et al. (2009) The Journal of biological chemistry 284, 24242-56. PMID: 19556245

6. Cai, Y. et al. (2010) The Journal of biological chemistry 285, 4268-72. PMID: 20018852

7. Song, JJ. et al. (2008) The Journal of biological chemistry 283, 35258-64. PMID: 18840606

8. Avdic, V. et al. (2011) Structure (London, England : 1993) 19, 101-8. PMID: 21220120

9. Zhang, P. et al. (2012) Nucleic acids research 40, 4237-46. PMID: 22266653

Note

For research use only.

Images

Flow Cytometry

Flow-cytometry using anti-human WDR5 antibody.MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human WDR5 antibody monoclonal antibody (Catalog # HX013013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human WDR5 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human WDR5 antibody monoclonal antibody (Catalog # HX013013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human WDR5 IP Antibody (SAA0239).

References

Datasheet

Anti-Human WDR5 IP Recombinant Antibody (SAA0239).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human WDR5 IP Recombinant Antibody (SAA0239) (HX013013)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us