Anti-Human GAPDH Monoclonal Antibody (1A200) (HY030035)

Name: Anti-Human GAPDH Monoclonal Antibody (1A200)
Brand: abinScience
SKU: HY030035
Price: 76 USD
Availability: InStock

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Overview

Catalog No.	HY030035
Description	Anti-Human GAPDH Monoclonal Antibody (1A200) [1A200] (HY030035) is a mouse monoclonal antibody detecting GAPDH in ELISA, WB. Suitable for Human, Mouse, and Rat. Highlights ●Recombinant — Superior batch-to-batch reproducibility with defined sequences. ●Multi-Species — Cross-reactive for translational research.
Species reactivity	Human, Mouse, Rat
Applications	ELISA, WB
Host species	Mouse
Clonality	Monoclonal
Isotype	IgG2b
Clone ID	1A200
Immunogen	E. coli - derived recombinant Human GAPDH protein (Met1-Glu335).
Target	GAPD, Peptidyl-cysteine S-nitrosylase GAPDH, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
RRID	Anti-Human GAPDH Monoclonal Antibody (1A200) (abinScience Cat# HY030035, RRID:AB_3751562)
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a ~36 kDa protein. Catalyzes the conversion of D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate in glycolysis and the reverse reaction in gluconeogenesis. Also shows nitrosylase activity, thereby playing a role in nuclear functions. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. 1. Tisdale, EJ. (2002) The Journal of biological chemistry 277, 3334-41. PMID: 11724794 2. Ercolani, L. et al. (1988) The Journal of biological chemistry 263, 15335-41. PMID: 3170585 3. Arif, A. et al. (2012) Molecular and cellular biology 32, 5046-55. PMID: 23071094 4. Gao, X. et al. (2013) Cell host & microbe 13, 87-99. PMID: 23332158 5. Gao, X. et al. (2016) The Journal of biological chemistry 291, 18232-8. PMID: 27387501
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Anti-Human GAPDH Monoclonal Antibody (1A200)
Western blot

Western blot analysis was performed using anti-GAPDH monoclonal antibody at 1ug/mL on various samples.
Lane 1: MCF-7 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: NIH3T3 cell lysate
Lane 4: PC-12 cell lysate
Lane 5: PC-3 cell lysate
Lane 6: Mouse kidney lysate
Lane 7: Rat spleen lysate
Lane 8: Rat kidney lysate