Anti-Human CUL1 Polyclonal Antibody (HC489014)

Name: Anti-Human CUL1 Polyclonal Antibody
Brand: abinScience
SKU: HC489014
Price: 96 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HC489014

Description

Anti-Human CUL1 Polyclonal Antibody (HC489014) is a rabbit polyclonal antibody detecting CUL1 in ELISA, IHC, WB. Suitable for Mus musculus and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CUL1 (Met1-Glu250).

Target

CUL1

Purification

Purified by antigen affinity column.

Accession

Q13616

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

Cullin-1, CUL-1, CUL1

Background

Cullin-1 (CUL1) is a ~89 kDa protein. Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. SCF complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and exchange of the substrate recognition component is mediated by TIP120A/CAND1.

1. Gao, D. et al. (2011) Molecular cell 44, 290-303. PMID: 22017875

2. Duan, S. et al. (2011) Molecular cell 44, 317-24. PMID: 22017877

3. Scott, DC. et al. (2016) Cell 166, 1198-1214.e24. PMID: 27565346

4. Liwocha, J. et al. (2024) Nature structural & molecular biology 31, 378-389. PMID: 38326650

5. Min, KW. et al. (2003) The Journal of biological chemistry 278, 15905-10. PMID: 12609982

6. Pagan, JK. et al. (2015) Nature cell biology 17, 31-43. PMID: 25503564

7. Man, X. et al. (2015) European journal of cell biology 94, 162-72. PMID: 25704143

8. Hopf, LVM. et al. (2022) Nature structural & molecular biology 29, 854-862. PMID: 35982156

10. Muhar, MF. et al. (2025) Nature 638, 519-527. PMID: 39880951

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CUL1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse brain lysate
Lane 2: Rat brain lysate

References

Datasheet

Anti-Human CUL1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human CUL1 Polyclonal Antibody (HC489014)

Overview

Images

References

Datasheet

SDS

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