Anti-Murine minute virus/MVM NS1 Polyclonal Antibody (VK011034)

Datasheet Share product Copy info

Price(USD): $

Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

VK011034

Description

Anti-Murine minute virus/MVM NS1 Polyclonal Antibody (VK011034) is a rabbit polyclonal antibody detecting NS1 in ELISA, IHC, WB. Suitable for Murine minute virus and Parvovirus LuIII.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Hamster parvovirus H1, Murine minute virus, Parvovirus LuIII

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Murine minute virus NS1 (Tyr6-Thr253).

Target

NS1

Purity

>90%

Purification

Purified by antigen affinity column.

Accession

P03134

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

Initiator protein NS1, NS1, EC:3.1.21.-, EC:3.6.4.12, NCVP1, Non-capsid protein NS-1, Non-structural protein NS1, NS1

Background

Initiator protein NS1 is a ~76 kDa protein. Multifunctional protein which displays endonuclease and helicase activities required for initiating and directing viral DNA replication. Also plays a role in viral packaging and transactivation of several promoters. Binds site-specifically to 2-3 approximate tandem copies of the tetranucleotide 5'TGGT3' within the origins of replication (Ori), unwinds this hairpin region and nicks one DNA strand thereby initiating the rolling circle replication (RCR). Cooperatively binds Ori with host PIF and probably other host factors, which activate the nickase function of NS1. Becomes covalently attached to the 5' end of the nick and provides a 3'OH for priming DNA synthesis.

1. Christensen, J. et al. (2002) Journal of virology 76, 6518-31. PMID: 12050365

2. Christensen, J. et al. (1995) Journal of virology 69, 5422-30. PMID: 7636987

3. Cotmore, SF. et al. (2007) Journal of virology 81, 13015-27. PMID: 17898054

4. Willwand, K. et al. (1997) The Journal of general virology 78 ( Pt 10), 2647-55. PMID: 9349487

5. Christensen, J. et al. (2001) Journal of virology 75, 7009-17. PMID: 11435581

Note

For research use only.

Images

References

Datasheet

Anti-Murine minute virus/MVM NS1 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Murine minute virus/MVM NS1 Polyclonal Antibody (VK011034)

Overview

Images

References

Datasheet

Quick access

Contact Us