Anti-Mouse 4-1BB/TNFRSF9/CD137 Recombinant Antibody (LOB12.3) (MC336013)

Name: Anti-Mouse 4-1BB/TNFRSF9/CD137 Recombinant Antibody (LOB12.3)
Brand: abinScience
SKU: MC336013
Price: 126 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

MC336013

Description

Anti-Mouse 4-1BB/TNFRSF9/CD137 Recombinant Antibody (LOB12.3) (MC336013) is a mouse monoclonal antibody detecting 4-1BB/TNFRSF9/CD137 in FCM, IF, ICC, ELISA, WB. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Mouse

Applications

FCM, IF, ICC, ELISA, WB

Host species

Mouse

Clonality

Monoclonal

Isotype

IgG2a, kappa

Clone ID

LOB12.3

Tested applications

Target

4-1BB ligand receptor, CD137, CDw137, ILA, T-cell antigen 4-1BB homolog, T-cell antigen ILA, TNFRSF9, Tumor necrosis factor receptor superfamily member 9

Endotoxin level

< 10 EU/mg

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P20334

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Tumor necrosis factor receptor superfamily member 9 (CD137/4-1BB) is a ~27 kDa protein. Receptor for TNFSF9/4-1BBL. Conveys a signal that enhances CD8(+) T-cell survival, cytotoxicity, and mitochondrial activity, thereby promoting immunity against viruses and tumors.

Note

For research use only

Isotype Control

Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)

Images

Western blot

Western blot analysis was performed using anti-CD137 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant mouse CD137 (Catalog No: MC336012)
Lane 2: negative control
Immunofluorescence

Immunofluorescence staining of HDLM2 with Mouse monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the cell membrane.