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Anti-Human SLC29A1 Polyclonal Antibody (HA253014)

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Overview
Catalog No.HA253014
Description
Anti-Human SLC29A1 Polyclonal Antibody (HA253014) is a rabbit polyclonal antibody detecting es-type; Solute carrier family 29 member 1; SLC29A1; ENT1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant human SLC29A1 (Asn30-Asn82&Arg228-Val291).
Target Equilibrative nucleoside transporter 1; hENT1; Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter; Equilibrative NBMPR-sensitive nucleoside transporter; es nucleoside transporter; Nucleoside transporter, es-type; Solute carrier family 29 member 1; SLC29A1; ENT1
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q99808
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Equilibrative nucleoside transporter 1 (SLC29A1) is a ~50 kDa protein. Uniporter involved in the facilitative transport of nucleosides and nucleobases, and contributes to maintaining their cellular homeostasis. Functions as a Na(+)-independent transporter. Involved in the transport of nucleosides such as adenosine, guanosine, inosine, uridine, thymidine and cytidine. Also transports purine nucleobases (hypoxanthine, adenine, guanine) and pyrimidine nucleobases (thymine, uracil). Mediates basolateral nucleoside uptake into Sertoli cells, thereby regulating the transport of nucleosides in testis across the blood-testis barrier.

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8. Aseervatham, J. et al. (2015) PloS one 10, e0136779. PMID: 26406980
9. Huang, W. et al. (2017) Protein & cell 8, 284-295. PMID: 27995448
10. Niemann, B. et al. (2022) Nature 609, 361-368. PMID: 35790189
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
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V₁ (Stock Vol.)
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