Anti-Human HARS1/jo-1 Recombinant Antibody (SAA2345) (HY584023)

Name: Anti-Human HARS1/jo-1 Recombinant Antibody (SAA2345)
Brand: abinScience
SKU: HY584023
Price: 126 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HY584023

Species reactivity

Human

Applications

ELISA, WB

Host species

Human

Isotype

IgG1

Clone ID

SAA2345

Clonality

Monoclonal

Target

Histidine--tRNA ligase cytoplasmic, EC:6.1.1.21, Histidyl-tRNA synthetase, HisRS, HARS1, HARS, HRS, jo-1

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P12081

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Histidine--tRNA ligase, cytoplasmic (HARS1/jo-1) is a ~57 kDa protein. Catalyzes the ATP-dependent ligation of histidine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP). Plays a role in axon guidance.

1. Abbott, JA. et al. (2018) Human mutation 39, 415-432. PMID: 29235198

2. Safka Brozkova, D. et al. (2015) Brain : a journal of neurology 138, 2161-72. PMID: 26072516

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-HARS1 monoclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: 293T cell lysate
Lane 3: K562 cell lysate
Lane 4: HepG2 cell lysate
Lane 5: HCT116 cell lysate
Lane 6: Jurkat cell lysate