Anti- Influenza A virus (H3N2) HA/Hemagglutinin Recombinant Antibody (S139) (VK515073)

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

VK515073

Species reactivity

Influenza A virus (H3N2)

Applications

ELISA

Host species

Human

Isotype

IgG1, kappa

Clone ID

H3N2

Clonality

Monoclonal

Target

Hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, HA

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P03435

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Hemagglutinin is a ~63 kDa protein. Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane.

Note

For research use only

Images

SDS-PAGE

SDS-PAGE for Anti- Influenza A virus (H3N2) HA/Hemagglutinin Antibody (S139)
Bioactivity

Detects recombinant H3N2 HA (Catalog No: VK515371) in indirect ELISA
Western blot

Western blot analysis was performed using anti-/ monoclonal antibody at 1ug/mL on various samples.
Lane 1: ATGE7523 200ng
Lane 2: 1* loading buffer

References

Datasheet

Anti- Influenza A virus (H3N2) HA/Hemagglutinin Recombinant Antibody (S139).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti- Influenza A virus (H3N2) HA/Hemagglutinin Recombinant Antibody (S139) (VK515073)

Overview

Images

References

Datasheet

SDS

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