Anti-Human CD162/SELPLG Recombinant Antibody (SAA2392) (HC298013)

Name: Anti-Human CD162/SELPLG Recombinant Antibody (SAA2392)
Brand: abinScience
SKU: HC298013
Price: 126 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HC298013

Species reactivity

Human

Applications

ELISA, FCM

Host species

Mouse

Clonality

Monoclonal

Isotype

IgG2a, kappa

Clone ID

SAA2392

Target

P-selectin glycoprotein ligand 1, PSGL-1, Selectin P ligand, CD162, SELPLG

Endotoxin level

<10 EU/mg

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q14242

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

P-selectin glycoprotein ligand 1 (CD162/SELPLG) is a ~43 kDa protein. A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.

Note

For research use only

Isotype Control

Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)

Images

SDS-PAGE

SDS-PAGE for Anti-Human CD162/SELPLG Antibody (SAA2392)
Flow cytometry

Flow-cytometry using APC anti-human CD162 antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD162 monoclonal antibody (Catalog HC298013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using PE anti-human CD162 antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD162 monoclonal antibody (Catalog HC298013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using FITC anti-human CD162 antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD162 monoclonal antibody (Catalog HC298013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.