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Anti-Mouse IL-13 Polyclonal Antibody (MW632014)

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Overview
Catalog No.MW632014
Description
Anti-Mouse IL13 Polyclonal Antibody (MW632014) is a rabbit polyclonal antibody detecting Il13. Suitable for Mouse.
Species reactivityMouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Mouse IL13 (Ser26-Phe131).
Target IL-13, IL13, Interleukin-13, NC30
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Accession P20109
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Interleukin-13 (IL13) is a ~14 kDa protein. Cytokine that plays important roles in allergic inflammation and immune response to parasite infection. Synergizes with IL2 in regulating interferon-gamma synthesis. Stimulates B-cell proliferation, and activation of eosinophils, basophils, and mast cells. Plays an important role in controlling IL33 activity by modulating the production of transmembrane and soluble forms of interleukin-1 receptor-like 1/IL1RL1. Displays the capacity to antagonize Th1-driven proinflammatory immune response and downregulates synthesis of many proinflammatory cytokines including IL1, IL6, IL10, IL12 and TNF through a mechanism that partially involves suppression of NF-kappa-B.

1. Finkelman, FD. et al. (2004) Immunological reviews 201, 139-55. PMID: 15361238
2. Zhang, M. et al. (2021) Journal of immunology (Baltimore, Md. : 1950) 207, 3070-3080. PMID: 34789557
3. Mayer, JU. et al. (2021) Nature immunology 22, 1538-1550. PMID: 34795444
4. Takeda, K. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 157, 3220-2. PMID: 8871614
5. Duffen, J. et al. (2018) Journal of immunology (Baltimore, Md. : 1950) 200, 1347-1359. PMID: 29305434
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Mouse IL-13 Polyclonal Antibody

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