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Anti-Human ADGRG3 Polyclonal Antibody (HA966024)

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Overview
Catalog No.HA966024
Description
Anti-Human ADGRG3 Polyclonal Antibody (HA966024) is a rabbit polyclonal antibody detecting ADGRG3. Suitable for Human.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human ADGRG3 (2*(Gly396-Thr434)).
Target ADGRG3, ADGRG3 C-terminal fragment, ADGRG3 N-terminal fragment, Adhesion G protein-coupled receptor G3, Adhesion G-protein coupled receptor G3, C-terminal fragment, Adhesion G-protein coupled receptor G3, N-terminal fragment, G-protein coupled receptor 97, GPR97, PGR26
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q86Y34
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Adhesion G protein-coupled receptor G3 (ADGRG3) is a ~60 kDa protein. Adhesion G-protein coupled receptor (aGPCR) for glucocorticoid hormones such as cortisol, cortisone and 11-deoxycortisol. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase. ADGRG3/GPR97 is coupled to G(o)/GNAO1 G proteins and mediates signaling by inhibiting adenylate cyclase activity. May also signal through G-alpha(q)-proteins; additional evidence are however required to confirm this result in vivo. Plays a role in the regulation of various processes including B-cell development, inflammation or innate immunity.

1. Ping, YQ. et al. (2021) Nature 589, 620-626. PMID: 33408414
2. Gupte, J. et al. (2012) FEBS letters 586, 1214-9. PMID: 22575658
3. Hsiao, CC. et al. (2018) Frontiers in immunology 9, 2830. PMID: 30559745
4. Chu, TY. et al. (2022) Nature communications 13, 6385. PMID: 36302784
5. Valtcheva, N. et al. (2013) The Journal of biological chemistry 288, 35736-48. PMID: 24178298
Note For research use only
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Formula
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Anti-Human ADGRG3 Polyclonal Antibody

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