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Anti-CD235a/GYPA (type N) Recombinant Antibody (N92) (HF002053)

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Overview
Catalog No.HF002053
Description
Anti-CD235a/GYPA (type N) Recombinant Antibody (N92) (HF002053) is a mouse monoclonal antibody detecting CD235a/GYPA in ELISA, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, WB
Host speciesMouse
ClonalityMonoclonal
IsotypeIgG2a, kappa
Clone IDN92
Target CD235a, GPA, GYPA, Glycophorin-A, MN sialoglycoprotein, MNS, PAS-2, Sialoglycoprotein alpha
Endotoxin level < 10 EU/mg as determined by LAL method.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P02724
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Glycophorin-A (CD235a/GYPA) is a ~16 kDa protein. Component of the ankyrin-1 complex, a multiprotein complex involved in the stability and shape of the erythrocyte membrane. Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane.

1. Vallese, F. et al. (2022) Nature structural & molecular biology 29, 706-718. PMID: 35835865
Note For research use only.
Isotype Control Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207)
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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