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Anti-SEOV Glycoprotein Gc Polyclonal Antibody (VK169034)

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Overview
Catalog No.VK169034
Description
Anti-SEOV Glycoprotein Gc Polyclonal Antibody (VK169034) is a rabbit polyclonal antibody detecting Glycoprotein Gc in ELISA, IHC, WB. Suitable for Seoul virus (SEOV).
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivitySeoul virus (SEOV)
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant SEOV GP (Trp655–Ile1068).
Target Envelopment polyprotein, GP, Glycoprotein 2, Glycoprotein C, Glycoprotein precursor, Gc, M polyprotein
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Accession P28729
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Envelopment polyprotein is a ~130 kDa protein. Structural component of the virion that interacts with glycoprotein C. It shields the hydrophobic fusion loops of the glycoprotein C, preventing premature fusion. The glycoprotein protrusions are arranged on an icosahedral lattice, with T=12 triangulation. They are able to attach the virion to the host cell receptor CD209/DC-SIGN and to promote fusion of membranes with the late endosome after endocytosis of the virion. Plays a role in the packaging of ribonucleoproteins and polymerase during virus assembly.

1. Freiberg, AN. et al. (2008) Journal of virology 82, 10341-8. PMID: 18715915
2. Halldorsson, S. et al. (2018) Nature communications 9, 349. PMID: 29367607
3. Lozach, PY. et al. (2011) Cell host & microbe 10, 75-88. PMID: 21767814
4. Piper, ME. et al. (2011) PloS one 6, e18070. PMID: 21445316
Note For research use only.
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References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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