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Anti-pan-Ebola virus Nucleoprotein Antibody (SAA2995) (VK059023)

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Overview
Catalog No.VK059023
Description
Anti-pan-Ebola virus Nucleoprotein Antibody (SAA2995) (VK059023) is a monoclonal antibody detecting Nucleoprotein in ELISA. Suitable for Bundibugyo virus (BDBV), Tai Forest ebolavirus (TAFV) (Cote d'Ivoire Ebola virus), Zaire ebolavirus (ZEBOV) (Zaire Ebola virus).
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityBundibugyo virus (BDBV), Tai Forest ebolavirus (TAFV) (Cote d'Ivoire Ebola virus), Zaire ebolavirus (ZEBOV) (Zaire Ebola virus)
ApplicationsELISA
ClonalityMonoclonal
Clone IDSAA2995
Target Nucleoprotein
Endotoxin level < 10 EU/mg as determined by LAL method.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Form Liquid
Storage buffer 0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Nucleoprotein (NP) is a ~56 kDa protein. Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins.

Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-pan-Ebola virus Nucleoprotein Antibody (SAA2995)

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