Anti-Mouse Perforin Polyclonal Antibody (MB886014)

Name: Anti-Mouse Perforin Polyclonal Antibody
Brand: abinScience
SKU: MB886014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

MB886014

Description

Anti-Mouse Perforin Polyclonal Antibody (MB886014) is a rabbit polyclonal antibody detecting Perforin in ELISA, IHC, WB. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Mouse Perforin (Leu413-Trp554).

Target

Cytolysin, Lymphocyte pore-forming protein, P1, PFP, PRF1, Perforin-1

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by antigen affinity column.

Accession

P10820

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

Perforin-1 is a ~61 kDa protein. Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes.

1. Praper, T. et al. (2011) The Journal of biological chemistry 286, 2946-55. PMID: 20889983

2. Law, RH. et al. (2010) Nature 468, 447-51. PMID: 21037563

3. Stewart, SE. et al. (2014) The Journal of biological chemistry 289, 9172-81. PMID: 24558045

4. Vergelli, M. et al. (1997) Journal of immunology (Baltimore, Md. : 1950) 158, 2756-61. PMID: 9058810

5. Ando, K. et al. (1997) Journal of immunology (Baltimore, Md. : 1950) 158, 5283-91. PMID: 9164947

6. Thiery, J. et al. (2010) Blood 115, 1582-93. PMID: 20038786

7. Czystowska, M. et al. (2010) Journal of molecular medicine (Berlin, Germany) 88, 577-88. PMID: 20225066

8. Zhou, Z. et al. (2020) Science (New York, N.Y.) 368. PMID: 32299851

Note

For research use only

Images

References

Datasheet

Anti-Mouse Perforin Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mouse Perforin Polyclonal Antibody (MB886014)

Overview

Images

References

Datasheet

SDS