Mouse Perforin Recombinant Protein (N-His) (MB886012)

Name: Mouse Perforin Recombinant Protein (N-His)
Brand: abinScience
SKU: MB886012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	MB886012
Description	Mouse Perforin Recombinant Protein (N-His) (MB886012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE. by SDS-PAGE. Highlights ●Recombinant — Consistent lot-to-lot performance. ●High Purity — >90% as determined by SDS-PAGE. by SDS-PAGE for demanding downstream applications.
Expression system	E. coli
Accession	P10820
Protein length	Leu413-Trp554
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Mus musculus (Mouse)
Nature	Recombinant
Endotoxin level	Remove endotoxin and sterilization is required for cell assay.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	18.19 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Cytolysin, Lymphocyte pore-forming protein, P1, PFP, PRF1, Perforin-1
Background	Perforin-1 is a ~61 kDa protein. Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes. 1. Praper, T. et al. (2011) The Journal of biological chemistry 286, 2946-55. PMID: 20889983 2. Law, RH. et al. (2010) Nature 468, 447-51. PMID: 21037563 3. Stewart, SE. et al. (2014) The Journal of biological chemistry 289, 9172-81. PMID: 24558045 4. Vergelli, M. et al. (1997) Journal of immunology (Baltimore, Md. : 1950) 158, 2756-61. PMID: 9058810 5. Ando, K. et al. (1997) Journal of immunology (Baltimore, Md. : 1950) 158, 5283-91. PMID: 9164947 6. Thiery, J. et al. (2010) Blood 115, 1582-93. PMID: 20038786 7. Czystowska, M. et al. (2010) Journal of molecular medicine (Berlin, Germany) 88, 577-88. PMID: 20225066 8. Zhou, Z. et al. (2020) Science (New York, N.Y.) 368. PMID: 32299851
Note	For research use only

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Datasheet

Mouse Perforin Recombinant Protein (N-His).pdf

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Formula

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Mouse Perforin Recombinant Protein (N-His) (MB886012)

Overview

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References

Datasheet

SDS